Order Mnemonic:

Alk Phos

Dept Name:

CHEMISTRY

Transport:

Refrigerated, original container

Processing:

Centrifuge.
Separate from cells if testing is delayed more than 8 hours.

Stability After
Processing:

Refrigerated: 48 hours

Minimum Testing
Volume:

0.1 mL

Unacceptable Conditions:

Gross hemolysis

Reference Range:

000 min - 005 yrs 110-302
005 yrs - 010 yrs 110-341
010 yrs - 012 yrs 103-373
012 yrs - 014 yrs 83-382
014 yrs - 016 yrs 67-372
016 yrs - 150 yrs 38-126

Methodology:

Alkaline phosphatase catalyzes the transphosphorylation of p-nitrophenylphosphate to p-nitrophenol(p-NP) in the presence of the transphosphorylating buffer 2-amino-2-methyl-1-propanol.
The change in absorbance at 405 nm due to the formation of p-NP is directly proportional to the ALP activity.
Beckman LX20

CPT Codes:

84075

Performed:

Sun-Sat

Reported:

Varies

NCD/LCD ABN Required:

No