Dept Name:

SEND OUTS

Reference Lab:

ARUP

Ref. Lab Test No.:

0030056

Refer to:

ARUP Lab

Transport:

Plasma, frozen

Processing:

Centrifuge and pour off into transport tube.

Stability After
Processing:

Ambient: Unacceptable
Refrigerated: Unacceptable
Frozen: 2 weeks

Minimum Testing
Volume:

0.5 mL

Unacceptable Conditions:

Hemolyzed specimens. Serum, EDTA plasma.

Reference Range:

By report

Additional Info:

Microtiter plates coated with monoclonal anti-ADAMTS13 antibody are used to measure ADAMTS13 activity by Fluorescence Resonance Energy Transfer (FRET).

ADAMTS13 levels of less than 5% may be associated with either inherited (Upshaw-Schulman Syndrome) or acquired thrombotic thrombocytopenic purpura (TTP).

A variety of medical conditions may result in a mild to moderate deficiency of ADAMTS13 activity. Recent plasma exchange therapy may raise the observed ADAMTS13 activity.

The monoclonal anti-ADAMTS13 antibody is directed against the CUB domain; therefore, certain forms of ADAMTS13 (with mutations in the CUB domains) may not be equivalently measured due to reduced binding to the antibody.

Methodology:

Fluorescence Resonance Energy Transfer

CPT Codes:

85397

Performed:

Mon-Fri

Reported:

2-5 days

NCD/LCD ABN Required:

No